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KMID : 1134819970260010067
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Journal of the Korean Society of Food Science and Nutrition
1997 Volume.26 No. 1 p.67 ~ p.71
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Effect of Lignin and Hemicellulose on the Mutagenicity of IQ
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Sung Mi-Kyung
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Abstract
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Different concentrations of lignin and hemicellulose were preincubated with 0.01㎍ of 2-amino-3-methylimidazo[4,5-f]quinoline(IQ) at simulated gastrointestinal pH conditions. The Ames Salmonella assay using Salmonella typhimurium TA98 was performed to detect any changes in the mutagenicity of IQ in the presence of lignin or hemicellulose. IQ revealed very weak or no mutagenicity when it was preincubated at pH 2.1. However, there was a dose-dependent inhibition of IQ mutagenicity in the presence of lignin or hemicellulose at pH 5.4 and 6.6. The antimutagenic activities of fibers were not different from each other. Also, at lower concentrations of fibers, pH 5.4 was more effective in suppressing IQ mutagenicity, while 300㎍ of lignin or hemicellulose significantly reduced the mutagenicity of IQ regardless of pH conditions. These results suggest that at gastrointestinal pH conditions, both soluble and insoluble fibers inhibit mutagenicity of IQ by adsorption. Therefore, a possible mechanism for protective effect of fibers against cancer is due to their adsorption to mutagens in the gastrointestinal tract, and pH seems to be an important factor in the regulation of interactions between the fiber and mutagens.
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KEYWORD
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lignin, hemicellulose, 2-amino-3-methylimidazo ［4, 5-f］quinoline, mutagen
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